Coding

Part:BBa_K909009:Design

Designed by: Gintautas Vainorius   Group: iGEM12_ETH_Zurich   (2012-09-23)

cDNA of UV-B sensing protein UVR8 from Arabidopsis thaliana


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 76
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 91


Design Notes

Contains two illegal PstI sites.


Source

cDNA of UVR8 from Arabidopsis thaliana was provided by Roman Ulm, University of Geneva.

References

  • Heijde, M., & Ulm, R. (2012). UV-B photoreceptor-mediated signalling in plants. Trends in plant science, 17(4), 230–7.
  • Christie, J. M., Arvai, A. S., Baxter, K. J., Heilmann, M., Pratt, A. J., O’Hara, A., Kelly, S. M., et al. (2012). Plant UVR8 photoreceptor senses UV-B by tryptophan-mediated disruption of cross-dimer salt bridges. Science (New York, N.Y.), 335(6075), 1492–6.